Reporter plasmid construction

Genomic DNA was isolated from Acinetobacter sp. ADPWH_lux (Huang et al. 2005) with the genomic DNA purification protocol from Cold Spring Harbor (Green and Sambrook 2012). The SalA promoter region, salR with promoter and luxCDABE were amplified from the Acinetobacter sp. ADPWH_lux genomic DNA and cloned into pJET1.2 (Thermo Scientific), using primers shown in Table S2. The salA promoter digested from pJET1.2 with BamHI and EcoRI, introduced upstream of salR in pJET1.2 digested with BamHI and EcoRI to generate plasmid pSALAR. luxCDABE was excised from pJET1.2 with EcoRI and inserted between the salA promoter and salR region in the pSALAR plasmid, resulting in plasmid pLUX, which was transformed into E. coli strain XL-1 Blue MRF’. Plasmids were selected on carbenicillin (200 µg/mL) and 500 µM SA. The plates were photographed in the dark after auto-exposure in a Gel Doc XR + Gel Documentation System (Bio-Rad). The reporter plasmid containing the PsalA::luxCDABE:salR cassette was named pLUX.