Western Blot Detection of Protein Expression (siRNA Knock-Down Control)

For CAR knock-down validation we provide the mRNA expression levels of CYP2B6 as a target gene of CAR. PPARα and FXR protein expression levels following siRNA treatment in HepaRG cells were assessed by Western blot (see Figure S4 in Supplementary Material). Antibodies used: rabbit anti-human PPARα, (CAYMAN No. 101710, dilution 1:500), FXR (Abnova H00009971-M01, dilution 1:1,000), mouse anti-β-Actin (Sigma-Aldrich, A5441, 1:500), goat-anti-rabbit-IRD800 (Li-COR, 926-32214, 1:10,000), and goat-anti-mouse-IRD650 (Li-COR, 926-68074, 1:10,000) were used as fluorescently labeled secondary antibodies.