Statistical Analysis

The variability in the concentration of Fe-FC and Fe-TAFC within the technical and biological replicates was assessed by the coefficient of variation (Supplementary Tables S3, S4). The concentrations of Fe-FC and Fe-TAFC are presented as the mean ± standard error of mean (Supplementary Table S4). The diagnostic performance of LC-CASI-FTICR methodology was expressed as sensitivity and specificity for Fe-FC, and Fe-TAFC using the procedure described in literature (Trevethan, 2017; Supplementary Table S1). The statistical analyses were performed using Microsoft Excel 2016 (Microsoft Corporation, Redmond, WA, United States). Standard uptake values (SUVs) in the lungs of the imaged animals were calculated by PMOD software after the selection of corresponding region of interest (ROI) – the lungs.