Prime-Boost Immunization

Seventy-two (72) female BALB/c mice were divided into three experimental groups (n = 24). Animals in the rFPV_Sg–Se group were primed with 1 × 10⁷ PFU of rFPV_Sg–Se in 100 μL of PBS by the intramuscular route at day 0 and were boosted with the same dosage at day 21. The second group received 1 × 10⁶ PFU of FPV282E4 (FPV282E4) at days 0 and 21, and the third group received 100 μL of PBS on days 0 and 21. Serum sample were collected at days 1, 7, 14, 21, 28, 35, 42, and 49 post immunization to detect SIV and vector-specific antibodies by ELISA. The experimental design is shown in Fig. 3a.

Quantifying the serum antigen specific IgG titer in BALB/c mice following a prime-boost rFPV_sg–se immunization. a Schematic of the experimental design. Female BALB/c mice were used (n = 24/group). The mice were divided into three groups and immunized with rFPV_sg–se, FPV282E4, or PBS. b SIV gag-specific IgG titers, c SIV gp120-specific IgG titers, and d vector-specific antibody levels were measured in the serum by ELISA