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Chromatin Immunoprecipitation (ChIP) Assay
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Freshly isolated nuclei from mouse livers were cross-linked in formaldehyde. ChIP assay was performed with a ChIP assay kit (Millipore, Bellerica, MA, USA) as described previously18. Anti-HNF4α antibody (PP-H1415; R&D Systems) was used in immunoprecipitation. The ChIP assay real-time PCR primer pairs amplify the −57/+60 Csad chromatin region (relative to the transcriptional start site as +1).
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