Fly strains

All flies were raised at 25°C on standard medium in a 12 hr light/12 hr dark cycle at 50% relative humidity. Fly strains used in this study include wild-type Canton-S; dsx^GAL4-DBD (generated in this study); dsx^GAL4 (Rideout et al., 2010); elav^VP16-AD (Luan et al., 2006); vGlut^{OK371-dVP16-AD} denoted as vGlut^dVP16-AD (Gao et al., 2008); Gad1^p65-AD (Diao et al., 2015); UAS-TNT_G (Sweeney et al., 1995); UAS-dTrpA1 (Hamada et al., 2008); UAS-pStingerII denoted as UAS-nGFP (Barolo et al., 2000); UAS-2XEGFP (Halfon et al., 2002); UAS-DenMark (Nicolaï et al., 2010); UAS-nSyb::GFP (Estes et al., 2000); UAS-GCaMP6m (Chen et al., 2013); UAS>stop>myrGFP (Yu et al., 2010); UAS>stop>TNT and UAS>stop>dTrpA1^myc (von Philipsborn et al., 2011); Otd-nls:FLPo (Asahina et al., 2014); UAS-GABA_B-R1-RNAi (VDRC: 101440); UAS-GABA_B-R2-RNAi (VDRC: 1784); UAS-GABA_B-R3 (Bloomington: 26729); UAS-Lcch3-RNAi (VDRC: 37408) and UAS-Rdl-RNAi (Liu et al., 2007). All lines and transgenes were in a w⁺ background for behavioral studies.