3.8. BACE-1 Inhibition Assay

The assay was carried out according to the supplied manual. Briefly, to 1.02 mL of buffer 150 μL of 60–600 μM VA solution or of IN sample (diluted 1:10 or 1:100 in water) were added, followed by 300 μL of the substrate solution and 30 μL of enzyme solution. After 2 h incubation at 37 °C, 600 μL of the stop solution were added and the fluorescence emission at 405 nm (λex = 320 nm) was measured. For the reference value, 150 μL of water replaced the inhibitor solution. For determining the blank value, 30 μL of buffer replaced the enzyme solution.