Cell culture and transfection

TZ Tianpeng Zhang
FY Fangjun Yu
LG Lianxia Guo
MC Min Chen
XY Xue Yuan
BW Baojian Wu
ask Ask a question
Favorite

Hepa-1c1c7 cells were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). The cells were cultured in Minimum Essential Medium Alpha Basic supplemented with 10% FBS. Hepa-1c1c7 cells were seeded onto 12-well plates and transfected with indicated plasmids and/or siRNA (sequences summarized in Table S1) using JetPrime (Polyplus Transfection, Ill kirch, France) according to the manufacturer's protocol.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A