Reagents

Bevacizumab was purchased from Chugai Pharmaceutical Co., Ltd. (Tokyo, Japan) and dissolved in saline. Rat anti-mouse cluster of differentiation (CD)31 monoclonal antibody was purchased from DIANOVA Vertriebs Gesellschaft mbH (Hamburg, Germany). Anti-α-smooth muscle actin (α-SMA) was purchased from Abcam (Cambridge, MA, USA). Indigocarmine was purchased from Daiichi Sankyo Company (Tokyo, Japan). All other chemicals and reagents were purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA), Sigma-Aldrich; Merck KGaA (Darmstadt, Germany) and Wako Pure Chemical Industries, Ltd. (Osaka, Japan), unless otherwise specified.