In Vitro Platelet Aggregation

Platelet‐rich plasma from clean air– or e‐cigarette–exposed mice was activated with ADP (0.5–2.5 μmol/L) and U46619 (2 μmol/L). Platelet aggregation was measured by the turbidometric method using model 700 aggregometer (Chrono‐Log Corporation). We also performed these aggregation experiments with ADP (2.5 μmol/L) and U46619 (2 μmol/L) in the presence (3 minutes incubation) or absence of 5 nmol/L prostacyclin (PGI₂). Each experiment was repeated at least 3 times with blood pooled from at least 3 different groups (ie, at least 5 mice each) that were exposed to either e‐cigarettes or clean air.