Assay Procedure

First, 80 μL of sample or pretreated serum was mixed with 4 µL of cTnI, CKMB, and Myo probes for 10 min of pre-incubation. Probes were enriched with an external magnet, and 80 µL of the supernatant was discarded. The probes were re-dispersed and dripped onto the conjugated pad. Then, 80 µL of the working buffer was pipetted onto the sample pad to facilitate probe migration along the strip by capillary action. After 10 min, another 30 µL of working buffer was applied to wash the background for 3 min. The strip was installed with a cartridge and inserted into the MIR apparatus for T and C line magnetic signals detection. By substituting the obtained T/C value into the calibration curve, the corresponding concentration of analytes can be obtained.