Cell apoptosis assay

In accordance with the manufacturer's protocol, SGC-7901 cell apoptosis was analyzed using the Annexin V-FITC/iodide (PI) cell apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA). The cells were washed with phosphate buffered salline (PBS) three times, followed by trypsin digestion, centrifugation at 400 × g at room temperature for 10 min, and were adjusted to 5×10⁴/ml, after which they were suspended in binding buffer containing Annexin V-FITC and PI. The fluorescence intensity was measured via flow cytometry (BD Biosciences) after 10 min of incubation in the dark.