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Apoptotic cells from larvae in each group were detected by staining with acridine orange. In brief, after psoralen treatment (at 96 hpf), the larvae were incubated with 10 μg/mL acridine orange staining solution in the dark for 30 min. They were then washed with PBS three times and anesthetized with 0.16% tricaine. A lateral image of each larva was acquired within 20 min using a fluorescence microscope.
Copyright and License information: ©2018 Xia, Wei, Zhang, Kong, Shi, Wang, Chen, Han and Liu.
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