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MTT Assay

SM Sven de Mey
HJ Heng Jiang
CC Cyril Corbet
HW Hui Wang
ID Inès Dufait
KL Kalun Law
EB Estelle Bastien
VV Valeri Verovski
TG Thierry Gevaert
OF Olivier Feron
MR Mark De Ridder
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Cytotoxicity of metformin and phenformin was assessed by MTT assay as described elsewhere (Wang et al., 2017). Briefly, after treatment, medium was aspirated and 50 μl MTT solution (5 mg/ml) was added for 1.5 h. Afterward, 200 μl of MTT solvent (19:1 DMSO: HCL) was added and admixed to dissolve the formazan crystals generated inside of cells. Absorbance was measured at a wavelength of 540 nm by using a spectrophotometer (Bio-Rad, CA, United States). Cell viability was determined by dividing the absorbance values of treated cells to that of untreated (control) cells.

Copyright and License information:  ©2018 de Mey, Jiang, Corbet, Wang, Dufait, Law, Bastien, Verovski, Gevaert, Feron and De Ridder.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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