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THP-1 cells were plated and differentiated as previously described. Cells were stimulated with LPS and IFNγ in the presence of Takinib or DMSO. 24 h after treatment, supernatant was added to ELISA plates (IL-6, IL-8, TNF, IL-1B, Ready- SET-Go! from Thermo). ELISA was conducted in accordance with the manufacturer’s protocol. Absorbance was measured at 450 and 570 nm. Experiments were repeated 3 times.
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