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Paraffin blocks of heart slices were cut in 6 μm-thick slices and stained with Masson’s trichrome for the evaluation of cardiomyocyte area. Digital images of transversally cut cardiomyocytes were captured using a light microscope (Olympus, Germany) at 20× magnification. Four mice were employed for each condition (three fields per heart), and cardiomyocyte size was calculated by quantitation of 150–200 cells per field using image analysis software (ImageJ).
Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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