4.8. Serum ELISA for inflammatory markers

Serum cytokine and chemokine levels were determined using V-PLEX Proinflammatory Panel 1 Mouse Kit (Meso Scale Discovery, Rockville, MD) according to manufacturer’s instructions. This kit measures mouse IFN-ɣ, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO (CXCL1), IL-10, IL-12p70, and TNF-α protein levels. The assay was read on a Meso QuickPlex SQ 120 machine and the results were analyzed using MSD Discovery Workbench 4.0 software. IL-4 and IL-12p70 failed to reach a detectable range in the serum of most of the mice, and therefore the data were not analyzed for any experiments.