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UV and Fluorescence detection of Dityrosine

MG Miao Gu
DB David C. Bode
JV John H. Viles
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Dityrosine has a fluorescence maximum at 410 nm, using an excitation wavelength of 310 nm. Dityrosine emission was monitored between 300 and 500 nm using a 1 cm quartz cuvette (Hellma) and a Hitachi F-2500 fluorescence spectrophotometer.

UV absorbance was also used to monitor dityrosine production of the covalent dimer by monitoring the absorbance spectrum at 315 nm with an extinction coefficient of 5,000 M−1 cm−1 71,72, using a 1 cm quartz cuvette (Hellma). Difference spectra, with non-oxidized Aβ subtracted from each spectrum are presented.

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