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All glucuronidation assays were performed for 2 hours in the presence of 10 μg microsomal proteins in a previously reported assay buffer [11, 12]. Kinetic parameters were assessed in liver and kidney samples using substrate concentrations ranging from 1 to 350 μM. The enzyme kinetic model was selected as recommended [25], using the Sigma Plot 11.2 assisted by Enzyme Kinetics 1.3 program (SSI, San Jose, CA).
Copyright and License information: ©2018 Martin Perreault et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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