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Germination Analysis

AM Anna Manara
GD Giovanni DalCorso
AF Antonella Furini
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Sterilized seeds were germinated on MS agar medium supplemented with different concentrations of ABA (0, 20, 50, and 100 μM, for 2 and 4 days), mannitol (100 and 200 mM, for 2 days), and NaCl (100 mM for 3 days and 200 mM for 7 days). The plants were grown in the phytochamber under the controlled conditions described above. Approximately, 100 seeds of each genotype were sown on each plate and scored for germination. Each experiment was performed in triplicate.

Copyright and License information:  ©2016 Manara, DalCorso and Furini.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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