2.5. Enzyme-Linked Immunosorbent Assay for IL-17 and IL-10

LM Lei Ma
HX HaiBo Xue
TG Tianqin Gao
MG MeiLan Gao
YZ YuJie Zhang
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Serum was isolated within 30 minutes of blood collection and stored at −80°C for further measurement of cytokine levels. The serum concentrations of IL-17 and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, USA).

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