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Tissue Preparation and ELISA Measurement of InsP Levels
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Frozen tissue was weighed and homogenized by sonication in 10 μl/mg of tissue of Tris-HCl buffer (100 mM; pH 7.5) containing 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1% SDS. Homogenates were diluted 1:50 and InsP levels were assessed with the IP-One ELISA kit (Cisbio, Codolet, France) according to the manufacturer’s instructions. Mean intra-assay and inter-assay coefficients of variation were 4.68 and 4.83%, respectively.
Copyright and License information: ©2018 Zuena, Iacovelli, Orlando, Di Menna, Casolini, Alemà, Di Cicco, Battaglia and Nicoletti.
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