Intraperitoneal Glucose Tolerance Test (IPGTT)

Mice were transferred to clean cages and fasted overnight for approximately 16 hrs. Mice were weighed to calculate and record the volume of 20% glucose solution required (2g of glucose/kg body mass) for IP injections for IPGTT: Volume of IP glucose injection (ml) =10 X body weight (g). Mice were restrained with an approved restrainer device with the tail exposed to score the tip of the tail with a sterilized scalpel blade. The first drop of blood was discarded and a small drop of blood is placed on the test strip of an animal blood glucose meter (Abbott Alpha TRAK 2 Blood Glucose Monitoring System) to record the fasting glucose level (t=0). Appropriate amounts of glucose were injected into the peritoneum as previously determined. The blood glucose levels were measured at 15, 30, 60 and 120 minutes following glucose injections and recorded. At the end of the experimental session, mice were placed in a clean cage with food and water.