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2.4. Measurement of Thermal Hyperalgesia
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Thermal hyperalgesia was assessed by measuring the paw withdrawal latency to radiant heat stimuli after the Von-Frey tests. Mice were placed in elevated chambers on a Plexiglas floor and were acclimated to the testing environment for 30 minutes before the experiments. The radiant heat source (Ugo Basile Plantar Test 37370) was applied to the center of the plantar surface of the hind paw with at least 3 min intervals. The average withdrawal latency of the trials was recorded as the response latency.
Copyright and License information: ©2018 Changming Wang et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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