Crystallization of the AMMO1 FAb and data collection

Crystals of AMMO1 Fab were obtained using a NT8 dispensing robot and screening was done with Rigaku Wizard Precipitant Synergy block#2, Molecular Dimensions Proplex screen HT-96, Hampton Research Crystal Screen HT by the vapor diffusion method. Crystals used for diffraction data were grown in 16.75% PEG 400, 13.4% PEG 3350, 0.1M MgCl2, 0.1M Tris pH 8.5. Crystals were cryo-protected in solutions containing 30% molar excess of their original reagents and 20% Glycerol. Crystal diffracted to 1.6 Å (See Table S4). Data was collected at ALS 5.1 and 5.2 and processed using HKL2000 (Otwinowski and Minor, 1997).