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Cell lines and culture conditions

MV Mireya Paulina Velasquez
DT David Torres
KI Kota Iwahori
SK Sunitha Kakarla
CA Caroline Arber
TR Tania Rodriguez-Cruz
AS Arpad Szoor
CB Challice L. Bonifant
CG Claudia Gerken
LC Laurence J. N. Cooper
XS Xiao-Tong Song
SG Stephen Gottschalk
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The Ph-positive acute B lymphoblastic leukemia (ALL) cell line BV173 (German Collection of Microoganisms and Cell Cultures, Braunschweig, Germany) and Burkitt’s lymphoma cell lines Daudi and Raji (ATCC, Manassas, VA) were used as CD19-positive targets. The generation of firefly luciferase (ffLuc)-expressing BV173 (BV173.ffLuc) and Daudi (Daudi.ffLuc) cells were described previously22,23. K562 (chronic myelogenous leukemia, ATCC) and A549 (lung carcinoma, ATCC) cell lines were used as negative controls. All cell lines were grown in RPMI 1640 (Thermo Scientific). 293T cells (ATCC) were used for packaging retroviral vectors and grown in DMEM. All media was supplemented with 10–20% FBS (Thermo Scientific) and 2 mmol/L GlutaMAX-I (Invitrogen, Carlsbad, CA).

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