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Primary neuronal culture preparation and treatments
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Primary neurons were prepared as described previously49. 7–9 DIV neurons were used for the different treatments. CaAB was performed as described previously1,12. Briefly, cells were incubated for 10 min in Ca2+/Mg2+-free Hank’s balanced salt solution (HBSS), supplemented with 20 mM HEPES buffer, 0.5 mM MgCl2, and 0.4 mM MgSO4. Calcium was then added back for 10 min to a final concentration of 1.8 mM. Cells were washed after the different treatments, homogenized, and analyzed by WB.
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