JK12 was grown under aerobic or micro-aerobic conditions as described above to investigate the influence of oxygen on its algicidal activity. JK12 culture supernatant was extracted at different time-points (8, 12, 24, 48 h) that reflects different growth stages of JK12. Subsequently, 10% v/v of the extracted culture supernatant was added into an exponentially growing algal culture. The mixture was incubated under algae culturing condition for 24 h and the algicidal rate was examined as described above.
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