Strains are available upon request. Information in supplemental material: Table S1 contains primer names and sequences used in this study. Figure S1 describes the forHR alleles, and the generation of the for0 null and fordup alleles. Figure S2 shows verification of the for0 allele. Figure S3 shows construction and verification of the forBAC construct. Figure S4 shows path length, food intake and fat levels of hemizygous rover and sitter larvae. Figure S5 shows foraging gene dosage and allelic contributions to foraging gene expression in whole larvae. Figure S6 provides foraging transcript sequences. File S1 provides the figure legends for Figures S1-S6.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.