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2.6. TUNEL Assay for Apoptosis in Cardiomyocyte
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TUNEL assays were performed to detect myocardial apoptosis using a commercial kit based on the manufacturer's instructions. The apoptotic cell nuclei was stained green and normal nuclei stained blue. Six fields at a magnification of ×200 were randomly selected from each sample using an optical microscope. The calculation of apoptosis index (AI) was performed as the ratio of TUNEL-positive nuclei to the total number of nuclei stained in each field. Image-Pro Plus 6.0 software was employed in our data analysis.
Copyright and License information: ©2018 Xiangwei Lv et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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