2.5. Inflammation-Related Gene Expression Measurement Using qPCR Array

The expression of 84 inflammation-related genes was evaluated using quantitative real-time PCR array (SABiosciences, Valencia, CA) according to the instructions. Total RNA was isolated using the RNeasy Mini Kit and then was quantified and qualitied by measuring the absorbance at 260 and 280 nm. Total RNA was purified with DNase. cDNA synthesis was performed by reverse transcription of 20 ng total RNA as described for RT-PCR and then combined with the SYBR Green Master Mix in 96-well plates following the manufacturer's recommendations. Thermal cycling was performed using an ABI Prism SDS 7300 system (Applied Biosystems, Madrid, Spain).