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Lung collagen deposition was estimated by measuring the hydroxyproline (HYP) content of lung homogenates with a hydroxyproline assay kit (Nanjing Jiancheng Bioengineering Company, China) in accordance with the manufacturer’s protocol. The absorbance of each sample at 550 nm wavelength was read by a microplate reader. The results were expressed in µg HYP per g wet lung.
Copyright and License information: ©2016 Journal of Thoracic Disease. All rights reserved.
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