2.11. LPS-Induced Peritonitis in Mice

To generate peritoneal exudates, C57BL/6 mice (n = 10/group) were intraperitoneally injected with 1.0 ml of sterile 4% thioglycollate broth, as previously described [33, 34]. Right after, Cc-ME (0, 50, and 200 mg/kg) suspended in 0.5% sodium CMC was orally administered by gavage once per day for 5 days. Acute peritonitis was induced by intraperitoneal injection of 1.0 ml (10 mg/kg) of LPS (E. coli 0111:B4) on day 4. On day 5, peritoneal fluid was collected by peritoneal lavage using sterile PBS. The effect of Cc-ME on peritonitis was examined by counting total leukocytes in a Neubauer chamber after staining with Turk solution.