FITC-Annexin V/PI apoptosis assay

Pancreatic cancer cell SW-1990 and PANC-1 were treated with dFv-LDP (2 nM), dFv-LDP-AE (0.001 nM) and dFv-R-LDP-AE (0.001 nM) for 24 h, and then the cells were collected and resuspended in 200 µl binding buffer. After that 10 µl FITC-labeled enhanced Annexin V and 100 ng propidium iodide were added. Upon incubation in the dark (15 min, room temperature), the samples were diluted with 500 µl binding buffer. Flow cytometry was carried out on a FACS Calibur (BD Company) and data were processed by its software.