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4.4. Induction the Model of Insulin—Resistant HepG2 Cells
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The model of insulin-resistant HepG2 cells was induced by PA. Briefly, a 100 mM PA stock solution was prepared in 0.1 M NaOH at 70 °C and then diluted in 10% (w/v) BSA solution to produce various concentrations of PA [53]. HepG2 cells were treated with PA (0, 150, 250, 350 μM) for 24 h in black 96-well plates, then cell glucose uptake was measured as mentioned to determine the optimum PA concentration used for building the insulin-resistant HepG2 cell model.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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