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Serum Hormone Analysis

HY Hyun Yang
SL Seung Yeon Lee
SL Sang R. Lee
BP Bo-Jeong Pyun
HK Hye Jin Kim
YL Young Ho Lee
SK Sun Woo Kwon
DS Dong Ho Suh
CL Choong Hwan Lee
EH Eui-Ju Hong
HL Hye Won Lee
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Blood samples were collected directly from the inferior vena cava using a 1-mL syringe at the end of the experiment. Serum was obtained by centrifugation at 2,000 × g for 10 min and stored at -70°C until use. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), and anti-Müllerian hormone (AMH) levels were measured using ELISA kits (Cusabio Biotech, Wuhan, China). Serum testosterone levels were measured using a Testosterone ELISA Kit (Abcam, Cambridge, United Kingdom). The 17β-estradiol (E2) levels were measured using an Estradiol (Rat) ELISA Kit (BioVision, United States). All hormone levels were measured according to the manufacturer’s instructions.

Copyright and License information:  ©2018 Yang, Lee, Lee, Pyun, Kim, Lee, Kwon, Suh, Lee, Hong and Lee.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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