4.5. Statistical Analysis

Data presented in the study are expressed as the mean value ± SEM of three separately performed experiments. The pooled datasets were checked for normality distribution by Shapiro-Wilk test and compared by nonparametric statistics methods (Friedman ANOVA and Kendall coefficient of concordance; Kruskal-Wallis ANOVA). The software package Statistica 13.3 (TIBCO Software Inc., Palo Alto, CA, USA) was used and differences were considered significant when the p value was <0.05. For the drawing of the Sankey diagram Flourish studio was used (Flourish Studio, Kiln Enterprises Ltd., London, UK).