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Xenograft model antitumor assay

YJ Yinghua Jin
LW Lingxiao Wei
QJ Qiuying Jiang
XS Xiaowei Song
CT Chong Teng
CF Chengjuan Fan
YL Yanju Lv
YL Ying Liu
WS Weixi Shen
LL Li Li
DH Dayong Huang
TX Tao Xin
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Three hundred CM-Dil labeled A549 xenograftzebrafish embryos were randomly chosen and seeded into 10 replicate wells in 6-well cell culture plates (30 embryos per well). Different concentrations of bevacizumab (28 ng, 83 ng and 250 ng per embryo), apatinib (0.057 μg/mL, 0.167 μg/mL and 0.5 μg/mL) and endostar (11 ng, 33 ng and 99 ng per embryo) were applied. After incubation for 3 days at 35 °C, ten out of 30 embryos from each well were randomly chosen and photographed under a fluorescence microscope. The fluoresce intensity of tumor in the embryos (Z) was measured and tumor inhibition rate was calculated according to the following formula:

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