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Preparation of S. crispa mycelial extracts

RK Ryoiti Kiyama
YF Yoshiyuki Furutani
KK Kayoko Kawaguchi
TN Toshio Nakanishi
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The aqueous extract of S. crispa mycelia (SCE) was prepared by boiling 50 g of the S. crispa powder in 1,000 ml of Milli-Q water for 10 min. The aqueous fraction was recovered by centrifugation at 3,300 × g for 25 min and sterilized by filtration through 0.22-μm filters (Millipore; Billerica, MA). To prepare the ethylacetate fraction (SCE-EtOAc), 100 ml of 10 mg/ml SCE was mixed with 100 ml of ethylacetate, and SCE-EtOAc was recovered as the upper fraction after centrifugation at 7,000 × g for 10 min. SCE-EtOAc was then dried by evaporation. The dried material dissolved in methanol was centrifuged at 3,300 × g for 10 min at 25 °C to remove debris, and the final concentration was adjusted to 10 mg/ml.

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