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2.6. Alkaline Phosphatase Activity (ALP) Analysis

AM Ashraf Al Madhoun
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A total number of 4 × 103 DPPSCs were cultured in each well of 24-well plates with the corresponding pre-conditioned media (BD, MZ, MTZ or control). At days 7 and 10, the ALP Staining kit (CosmoBio, Carlsbad, CA, USA) was used in accordance with the manufacturer’s instructions. ALP- staining was observed and cell images were taken on optical microscope (Euromex, Arnhem, The Netherlands). Alkaline phosphatase activity was quantified by spectrophotometry and the absorbance was measured at 405 nm for a period of 1 and 2 min.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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