Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Bronchoalveolar lavage fluid (BALF)
Request a Protocol
Ask a
question
Favorite
We collected BALF by lavage the lung for three times with PBS (pH 7.2, 500 μL each time). The fluid recovery rate was more than 90%. Lavage samples from mice were kept on ice. BALF was centrifuged at 700× g for 5 min at 4 °C. The BALF supernatant was collected and stored at −80 °C.
Copyright and License information: ©2018 Lu et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
