2.6. Histology and Oil Red O staining of liver sections

Mukund P. Srinivasan; Noha M. Shawky; Bhupendra S. Kaphalia; Muthusamy Thangaraju; Lakshman Segar

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2.6. Histology and Oil Red O staining of liver sections

MS Mukund P. Srinivasan

NS Noha M. Shawky

BK Bhupendra S. Kaphalia

MT Muthusamy Thangaraju

LS Lakshman Segar

This method is extracted from research article: Biochem Pharmacol, Dec 2018

Alcohol-induced ketonemia is associated with lowering of blood glucose, downregulation of gluconeogenic genes, and depletion of hepatic glycogen in type 2 diabetic db/db mice

DOI: 10.1016/j.bcp.2018.12.005

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The liver sections were stained with Hematoxylin and Eosin (H&E) and Oil Red O to evaluate lipid accumulation in hepatocytes and examine the extent of steatosis [39, 40]. The images of the liver sections were captured under bright field with Olympus DP72 microscope BX43 using 40X magnification. The total area of lipid droplets was analyzed using image J software (version 1.50i, Bethesda, NIH) [39].

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