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Dorsal Root Ganglion Neurons (DRG) Co-culture for Myelination Assays
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Similar to OPCs in mice, DRGs were also difficult to prepare, so we have to resort to their rat counterpart as substitutes. DRGs were isolated from E15 Sprague-Dawley rat embryos as previously described (Dadsetan et al., 2009), and cultured for neurite outgrowth as described by Dincman et al. (2012). OPCs/DRGs co-cultures were maintained 8–12 days in OPC basal media supplemented with 30 ng/ml Triiodothyronine (T3; Sigma), and fixed for immunostaining with anti-MBP and anti-neurofilament (axons) antibodies.
Copyright and License information: ©2016 Yang, Cheng, Shen, Xie, Zhao, Zhang, Cao, Shen and Qiu.
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