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The human colon adenocarcinoma cell line, SW480, was purchased from American Type Culture Collection (Manassas, VA). SW480 colon cancer cells were grown as monolayers (37°C in 5% CO2) in DMEM (Invitrogen Life Technologies, Carlsbad, CA) supplemented with 10% FBS (GIBCO) and 1% penicillin-streptomycin. Cells were detached and prepared as previously described (32).
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