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4.4. Determination of Triglyceride (TG) in the Liver
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Total lipids were extracted from a homogenate prepared from 200 mg of a mouse liver in a chloroform/methanol mix (2:1, v/v). TG levels in the total lipid extract were determined enzymatically using a commercially available enzymatic kit (Sigma Chemical Co., St. Louis, MO, USA) according to the manufacturer′s protocol. In addition, to evaluate lipid staining in the liver, 5 µm-thick cross sections of the left lateral lobe of the liver were immersed in propylene glycol and stained with Oil Red O reagent.
Copyright and License information: ©2018 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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