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NOD2, ATG16L1 and IRGM genotyping

GB Guillaume Le Baut
CO Claire O'Brien
PP Paul Pavli
MR Maryline Roy
PS Philippe Seksik
XT Xavier Tréton
SN Stéphane Nancey
NB Nicolas Barnich
MB Madeleine Bezault
CA Claire Auzolle
DC Dominique Cazals-Hatem
JV Jérome Viala
MA Matthieu Allez
JH Jean-Pierre Hugot
AD Anne Dumay
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Allelic discrimination assays were used to genotype patients and controls for five susceptibility variants: NOD2 SNPs rs2066844 (assay ID: C-11717468_20), rs2066845 (assay ID: C_11717466_20), and rs2066847 (custom assay); ATG16L1 SNP rs2241880 (assay ID: C_9095577_20); and IRGM SNP rs10065172 (assay ID: C_30593568_10). These SNPs were analyzed using real-time PCR as recommended by the manufacturer (Applied Biosystems, USA). Reactions were carried out using a LightCycler 480 Systems (Roche). An initial denaturation step was performed at 95°C for 10 min, followed by 40 cycles of 92°C for 15 s and 1 min at 60°C.

Copyright and License information:  ©2018 Le Baut, O'Brien, Pavli, Roy, Seksik, Tréton, Nancey, Barnich, Bezault, Auzolle, Cazals-Hatem, Viala, Allez, The REMIND GROUP, Hugot and Dumay.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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