5.7. Hemolytic Activity

Hemolytic activity of StigA6, StigA16, and Stigmurin was carried out by incubating a suspension of healthy human donor O⁺ erythrocytes with serially diluted concentrations of the peptides. Cells were first washed three times by centrifugation at 2000 RPM for 10 min in PBS, then incubated with the synthetic peptides (1.17–75 µM) at 37 °C for 1 h [45]. Optical density of supernatants was measured at 540 nm using a microplate reader. Triton was used as positive control, and no compost was added to the negative control for 0% hemolysis.