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Apoptosis assay using Annexin V/Propidium iodide staining

YA Yasmeen M. Attia
DE Dina M. El-Kersh
HW Hebatallah A. Wagdy
ME Mohamed M. Elmazar
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Caco-2 cells cultured in six-well plates were treated with 10 µM of 5-FU + 0.1 µM of VER for 48 hours. Then, the cells were fixed in cold ethanol for half an hour and stained with 5 μl Annexin V-FITC and 5 μl propidium iodide (PI) using an Annexin V-FITC Apoptosis Detection Kit (BioVision, CA, USA). The cells were then placed at room temperature for 15 min in the dark then analyzed by a FACScan flow cytometer (Beckman Coulter, CA, USA). Apoptosis was evaluated in terms of the FITC-positive cells.

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