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To determine cardiac fibrosis, we performed picrosirius red staining. In brief, 10% formalin fixed left-ventricular paraffin sections (5 μm) were processed for picrosirius red staining. The reagents were Direct Red 80 (cat # 365548) and picric acid (cat # P6744-1GA) from Sigma Aldrich, St. Louis, MO, United States and glacial acetic acid (cat # A38-500) from Thermo Fisher Scientific, Waltham, MA, United States. We used standard kit protocol for this staining. We used the Tissue Core facility of the University of Nebraska Medical Center for this staining.
Copyright and License information: ©2018 Nandi, Shahshahan, Shang, Kutty, Boska and Mishra.
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