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2.7. Microbiota Analysis
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Sample for bead-based sequencing was set up according to an earlier report [27] and sequenced using Roche 454 GS FLX platform on GS FLX instruments from Roche (Roche, Nutley, NJ, USA) [28]. The heat map of 16S rRNA gene sequences on the 21st day and 42nd day was created using Genomics Viewer at http://www.broadinstitute.org/igv. The R packages stats were used to perform statistical analysis. Simpson index and Shannon index were used to analyze the community diversity among different groups [29].
Copyright and License information: ©2018 Sitong Zhang et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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